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Volume: 7
Issue: 09
Date: 24-Sep-99


Table of Contents:

I.    LYMENET: Bachmann Bash '99 to Assist LymeNet Fundraising Efforts
II.   MED MICROBIOL IMMUNOL: Osp17, a novel immunodominant outer
      surface protein of Borrelia afzelii: recombinant expression
      in Escherichia coli and its use as a diagnostic antigen
      for serodiagnosis of Lyme borreliosis.
III.  BIOL PSYCHIATRY: Borrelia burgdorferi central nervous system
      infection presenting as an organic schizophrenialike disorder
IV.   ABOUT THE LYMENET NEWSLETTER


Newsletter:

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                  Volume 7 / Number 09 / 24-SEP-1999
                                INDEX



I.    LYMENET: Bachmann Bash '99 to Assist LymeNet Fundraising Efforts
II.   MED MICROBIOL IMMUNOL: Osp17, a novel immunodominant outer
     surface protein of Borrelia afzelii: recombinant expression
     in Escherichia coli and its use as a diagnostic antigen
     for serodiagnosis of Lyme borreliosis.
III.  BIOL PSYCHIATRY: Borrelia burgdorferi central nervous system
     infection presenting as an organic schizophrenialike disorder
IV.   ABOUT THE LYMENET NEWSLETTER



=====*=====


I.    LYMENET: Bachmann Bash '99 to Assist LymeNet Fundraising Efforts
----------------------------------------------------------------------
Sender: Marc Gabriel <a229@Lehigh.edu>
Date: September 21, 1999

The Fourth Annual Bachmann Bash for the Lyme Disease Network of New
Jersey will take place on Saturday, October 2, 1999, at St. Michael's
Church Hall in Paterson, NJ.   The Millennium Party will begin at
6:45 p.m.

The non-profit Lyme Disease Network (LymeNet), which brings you this
newsletter every month, obtains its operating budget from individual
donations.  Each fall, LymeNet embarks on a fundraising drive to secure
funds for the following calendar year.  LymeNet's series of Internet
services provides information to thousands of web visitors each day.

Ironically, LymeNet is becoming increasingly important as a new
generation of consumer health web sites proliferate on the Internet.  
Many of them contain outdated information on Lyme disease, often
ignoring the last decade of research.  Others use figures out of

context, clearly demonstrating the lack of commitment to the topic.  
Since 1994, LymeNet has been dedicated to ensuring the information
presented is as up-to-date as possible.

"The Lyme Disease Network helps people who are in desperate need," says
Angela Bachmann, who organizes the annual Bachmann Bash Fundraiser.
In 1996, Angela and her husband Lou searched desperately for answers
as their daughter Lori became increasingly incapacitated by an illness
no local physician could diagnose.  After finding LymeNet, they finally
had the missing pieces of information they needed to set Lori on her
path to recovery.


"The Bachmann Bash Fundraiser for the Lyme Disease Network is held
each year and includes all our family and friends," Angela notes.
"If we don't bring in adequate donations, the LDN will perhaps no
longer be available to us. "


For those who can't attend, individual donations are also accepted.
For more information on the fundraiser and donations, please visit:


      http://www2.LymeNet.org/domino/news.nsf/UID/bash99


=====*=====


II.   MED MICROBIOL IMMUNOL: Osp17, a novel immunodominant outer
     surface protein of Borrelia afzelii: recombinant expression
     in Escherichia coli and its use as a diagnostic antigen
     for serodiagnosis of Lyme borreliosis.
------------------------------------------------------------------
AUTHORS: Jauris-Heipke S, Rossle B, Wanner G, Habermann C, Rossler D
        Fingerle V, Lehnert G, Lobentanzer R, Pradel I, Hillenbrand B
Schulte-Spechtel U, Wilske B
ORGANIZATION: Max von Pettenkofer-Institut fur Hygiene und Medizinische
             Mikrobiologie, Ludwig-Maximilians-Universitat Munchen,
     Germany.
REFERENCE: Med Microbiol Immunol (Berl) 1999 May;187(4):213-9
ABSTRACT:


Western blot analyses of the human humoral response of patients with
Lyme borreliosis have shown that a 17-kDa protein is an immunodominant
protein in late disease. Immune electron microscopy with a monoclonal
antibody against this protein revealed that the 17-kDa protein is
abundantly expressed on the surface of Borrelia afzelii strain PKo.
Therefore, the protein has been renamed outer surface protein (Osp)
17. Recombinant Osp 17 of strain PKo was expressed in Escherichia coli
and purified by chromatography. Immunoblot analysis of human sera
showed a comparable sensitivity with recombinant and natural proteins.
The DNA sequences of the osp17 genes from different B. afzelii strains
were determined. The DNA sequences of the different osp 17 homologues
(six isolates from skin, three isolates from CSF and one isolate from
synovial fluid) had high sequence identities of at least 94%. Using a
polyclonal antibody against recombinant Osp 17, it was shown that
Osp 17 expression varied considerably among the investigated B.

afzelii strains. As previously also observed for OspA- and
OspC-encoding genes, the osp 17 gene is present in strains not
expressing the respective protein. It has been shown that OspA and
OspC expression varies in different environments such as tick and
vertebrate host. Studies are underway to examine whether this is
also true for Osp 17. For diagnostic purposes the use of recombinant
Osp 17 has the advantage that the amount of Osp 17 antigen can be
easily standardized for immunoblotting, and that this antigen can be
used in a protein-specific enzyme-linked immunosorbent assay.



=====*=====


III.  BIOL PSYCHIATRY: Borrelia burgdorferi central nervous system
     infection presenting as an organic schizophrenialike disorder
-------------------------------------------------------------------
AUTHORS: Hess A, Buchmann J, Zettl UK, Henschel S, Schlaefke D, Grau G
        Benecke R
ORGANIZATION: Department of Neurology, University of Rostock, Germany.
REFERENCE: Biol Psychiatry 1999 Mar 15;45(6):795
REFERENCE:


BACKGROUND: We report on a 42-year-old female patient who presented
with a schizophreniform disorder and complete relief of symptoms after
specific therapy.
METHODS: Cerebrospinal fluid and magnetic resonance imaging findings
led to the diagnosis of Lyme disease.
RESULTS: To our knowledge this is the first reported case with an
exclusive psychiatric manifestation of Lyme disease.
CONCLUSIONS: In case of first manifestation of psychotic disorder,
although neurological symptoms are lacking, Lyme disease should be
considered and be excluded by cerebrospinal fluid analysis



=====*=====


IV.   ABOUT THE LYMENET NEWSLETTER
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         William S. Stolow, President <bill_stolow@lymenet.org>
         The Lyme Disease Network of New Jersey
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